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KMID : 0545120110210010089
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 1 p.89 ~ p.92
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Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis
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Sanjay Kumar
Urmil Tuteja
Kumari Sarika
Dhirendra Kumar Singh
Ashok Kumar
Om Kumar
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Abstract
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The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratoryacquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.
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KEYWORD
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Multiplex PCR, Internal amplification control, Brucellosis
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